SingHealth Advanced Bioimaging aims to build up a bioimaging hub for both SingHealth and Singapore investigators by providing a wide variety of imaging platforms and services in both light and electron microscopy.

Our core values are
1. To provide guidance and expertise in image acquisition and analysis to investigators
2. To provide imaging services to SingHealth and Singapore investigators.
3. To educate and train investigators in bio-imaging techniques through conducting workshops, seminars, and hands-on training sessions.

To view the brochure of Advanced Bioimaging, please click here.

For a list of SingHealth Core Platforms Equipment, click here​.

To view the price list for services provided by Advanced Bioimaging, click here (only viewable by SingHealth staff with Intranet access).

Core Services

The Advanced Bioimaging Core Platform provides imaging services for both light and electron microscopy. It focuses on super-resolution light microscopy, capable of conducting five types of imaging techniques:

1) Structured Illumination Microscopy (SIM)
2) Stimulated Emission Depletion (STED) microscopy
3) Nikon-Stochastic Optical Reconstruction Microscopy (N-STORM)
4) Direct-Stochastic Optical Reconstruction Microscopy (d-STORM)
5) Photoactivated Localisation Microscopy (PALM).

Each super-resolution imaging technique adopts a unique methodology for improving the resolution beyond the diffraction limit for light microscopy. The table below summarises the lateral (XY) and axial resolutions that can be obtained with each technique.

 

 

The advantage of SIM and STED is their fast acquisition speed: images of cellular structures can be obtained in minutes. STORM and PALM are single molecule imaging techniques, which can obtain much higher resolution, but they require many thousands of frames to be collected. The X, Y and Z-coordinates of all detected molecules are then collected to form a single 3D image of the structure. Image acquisition and molecule detection typically takes 15 minutes.  

In addition to super-resolution imaging, the SingHealth Advanced Bioimaging Core Platform provides access services for many conventional imaging techniques in light microscopy:

1) Bright field (BF).
2) Differential Interference Contrast (DIC).
3) Wide-Field fluorescence (WF).
4) Total Internal Reflection Fluorescence (TIRF).
5)  Live-cell imaging (BF & WF),
6)  Laser scanning confocal microscopy (LSCM).
7)  High-Content Screening (HCS).

For image processing, the Core Platform provides two high-end HP Z420 workstations installed with the following scientific image rendering and analysis software:

1) NIS-Elements
2) ImageJ
3) Imaris
4) Huygens
5) LAS-AF Lite
6) MetaXpress
7) ZEN and ZEN lite

For electron microscopy, the core provides ultra-thin tissue sample preparation and imaging services for transmission electron microscopy (TEM). The core facility currently owns a Joel JEM1200 TEM and the-state-of-the-art EM preparation equipment from Leica. The EM preparation room is supplied with necessary chemicals and reagents for ultra-thin tissue sectioning, staining, coating and treatment. Below is a list of our equipment:

1) Leica EM KMR3 – Glass knife maker
2) Leica EM ACE200 Sputter coater
3) Leica EM CPD300 Critical Point Dryer
4) Leica EM AC20 Staining machine
5) Leica ULTRACUT EM UC7 Ultra microtome
6) Joel JEM1200 TEM

In addition, the core facility has a fully functioning cell culture room, equipped with a CO2 incubator, phase contrast microscope and a laminar flow hood for live cell/tissue treatment.

 

Application process for conducting imaging

Investigators who would like to use our imaging platforms are encouraged to have a discussion with the core staff regarding the microscopy platform, imaging technique and sample preparation conditions to be used so that the desired imaging results will be achieved. After that, a specific training or hands-on session can be arranged. Normally, it is desirable to give a one-week notice for the Core Platform staff to schedule the work. Finally, users need to assist the core staff with processing the paperwork for billing the training and/or usage charges.

 

Users are requested to conduct due diligence to ensure a safe and risk-free imaging environment, to maintain the working conditions of the optical equipment and to keep the microscopy room tidy and clean for the convenience of the Core Platform staff and other users.


The following list of platforms can be booked via our Google calendar:

1) Nikon N-STORM (BF, WF, TIRF, live cell, d-STORM & N-STORM)
2) Leica STED-3X (3D-STED and confocal)
3) ZEISS Elyra PS.1 (WF, TIRF, SIM, PALM & d-STORM)
4) Molecular Devices MetaXpress (HCS & live cell)
5) Image processing WS1 (NIS-Elements, Imaris, MetaXpres, ImageJ, ZEN lite, LAS-AF lite)
6) Image processing WS2 (NIS-Elements, ZEN, Huygens, ImageJ, ZEN lite, LAS-AF lite)
7) EM sample preparation lab (Glass knife maker, Sputter coater, Critical Point Dryer and Staining machine)
8) Leica ULTRACUT EM UC7 Ultra microtome
9) Joel JEM1200 TEM

 

Contact Details

The core is open to SingHealth and Singapore investigators. Training, imaging services and hands-on help are available upon request at a charge. For inquiries regarding imaging, training, and charging, please contact the core facility staff via email at bioimaging@singhealth.com.sg

Investigators are welcome to contact core facility management team for any collaboration possibilities:

SingHealth Advanced Bioimaging's Director: Antonius VanDongen via antonius.vandongen@duke-nus.edu.sg.