The Advanced Molecular Pathology Laboratory (AMPL) is a one-stop service for researchers and industry partners developing tissue arrays, from exploratory animal toxicological studies to biomarker discovery and validation of clinical material. A collaboration between the Institute of Molecular and Cell Biology (IMCB) and SingHealth, AMPL operates out of two campuses – AMPL@Biopolis at IMCB and AMPL@SingHealth at the Academia on Singapore General Hospital Campus.
Our College of American Pathologists (CAP)-compliant facilities provide:
- Technical support for tissue-based research
- High quality tissue bioresources for the research community and biomedical industry
To ensure the highest professional standards, AMPL has a team of diagnostic histopathologists, veterinary pathologists and scientists, supported by experienced medical and laboratory technologists. Its veterinary pathologists are board certified in toxicological pathology and have decades of experience of working with the pharmaceutical industry.
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AMPL@Biopolis specialises in animal research services, including animal necropsy, veterinary pathology, histology services and image analysis.
At AMPL@SingHealth, which is headed by an experienced qualified histopathologist, research partners will have access to a variety of techniques necessary for human tissue-based research from histopathology consultation to immunodiagnostics.
AMPL@SingHealth is closely linked with the SingHealth Tissue Repository (STR), which is the largest research tissue repository in Singapore.
AMPL provides a full-range of research services at cost-efficient prices, pegged below clinical laboratory charges for similar services.
Services provided span from advice during project design, applications to the Institutional Review Board (IRB) and tissue access committees, project management, and execution.
Pathology and histological consultation
- Consultation with a qualified pathologist supported by a team of pathology associates for:
- Interpretation of histology and immunostaining
- Classification and grading of neoplasms according to standard or protocol-defined criteria
- Selection of material for downstream applications
- Interpretation of histochemical and immunohistochemical stains
- Histological techniques, such as tissue processing, cryosectioning, microtomy, standard and special histochemical staining
- Academic post mortem examinations (upon request)
- Frozen section consultation for modern molecular techniques, such as DNA microarray, comparative genomic hybridisation (CGH) and SELDI-mass spectrometry
- Full range of immunodiagnostic techniques ranging from single-label immunostaining to multi-colour immunolabelling techniques.
- TUNEL assays for apoptosis and in situ hybridisation (ISH) assays for detection of viral RNA can also be performed.
In addition, STR can evaluate new antibodies for research and diagnostic purposes on cytological specimens, paraffin embedded archival material and tissue microarrays.
Tissue microarray construction
- Construction and stocking of tissue microarrays (TMA) derived from human tissues and neoplasm
- Custom-made tissue arrays
- Construction of cellular arrays derived from malignant effusions and cell lines
All tissue arrays are produced under the supervision of a consultant pathologist, ensuring quality.
Fluorescence in situ hybridisation
- Fluorescence in situ hybridisation (FISH)
- Whole chromosome painting with homemade and commercial reagents
- Immuno-FISH (FICTION technique), which combines the power of FISH to detect genetic alterations with lineage identification of cells of interest by immunophenotype
Cellular array construction
- Isolated cell preparations from paraffin embedded archival material, fresh tissues as well as cytological specimens for immunocytochemistry, FISH and immuno-FISH experiments are available
- Cellular arrays from a variety of human neoplasms are in stock.
- Laser microdissection by a trained pathologist using the Arcturus LCM, Leica LMD and Carl Zeiss' PALM systems
Nucleic acid extraction
- DNA and RNA extraction from frozen using Qiagen EZ1 XL Advanced Machine
- Calculation of the quantity of the extracted samples performed using Caliper DropSense Machine
- Spectrophotometry and gel electrophoresis, quality scoring for RNA and DNA performed using Caliper GX II machine
Antibody evaluation and probe-making
- Screening of antibodies and evaluating staining characteristics in normal and neoplastic tissues of human and animal origins,
- Design, construct and validate vlocus-specific FISH probes against genes of interests or region-specific probes to verify regions of amplifications/deletions as identified by CGH
For details of the services available at AMPL@Biopolis, please click here.
For researchers, medical students, clinicians and pathologists on attachment with AMPL@SingHealth, customised training is provided to meet specific needs of the attachment programme.
Training workshops include:
- Basic histotechnology – microtomy, haematoxylin/eosin staining, making cryostat sections
- Basic immunohistochemistry – immunostaining of frozen sections, paraffin embedded tissues and cytological specimens
- Multi-label immunohistochemistry – double and triple immunofluorescence labelling, combining immunoperoxidase with immunofluorescence staining
- FISH – performing FISH on metaphase spreads, blood smears and cytological specimens, frozen sections and paraffin embedded tissue sections
- Tissue microarray technology – constructing tissue microarrays from archival paraffin embedded material
- Laser microdissection
AMPL@SingHealth charges include two components: (1) reagent charge and (2) service charge.
The service charge depends on the nature of the protocol, the complexity of the procedure and the number of specimens. Contact Ms Magadalene Koh at email@example.com to enquire about the latest schedule of reagent charges.
To find out more about the services at AMPL@SingHealth, please contact Ms Magdalene Koh at firstname.lastname@example.org.